FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n02_CMV_S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n02_CMV_S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6894669
Sequences flagged as poor quality0
Sequence length80
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTG4750986.890802154534177No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAT4150976.020550080069109No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTT4063775.894075553155634No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAA4030955.84647355804898No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTA3930315.700505709556181No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGA3897255.6525556194213245No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAG3437414.985605545385862No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAC3298944.784769218072688No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCT3296694.781505827183292No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGT3203044.645676246386882No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTC2930064.249747159725869No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCA2735773.9679497304366604No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGG2488713.609614906821488No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCG2475783.5908612871770926No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGC2316613.3600017636814763No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCC2254123.2693665207133225No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG752821.0918870797133262No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCCTG381060.5526878810280813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG91200.13227611071684514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCC6252150.073.943221
CGATCCG6235800.073.761382
GATCCGA6281600.073.358483
ATCCGAC6313850.072.981644
TCCGACT6338150.072.63665
CGACTCG6342300.072.395617
GACTCGG6254650.072.31328
ACTCGGT6274650.072.133559
CCGACTC6374450.071.973936
CTCGGTG6274900.071.9515110
TCGGTGC6290500.071.8007111
CGGTGCC6258450.071.73871612
GGTGCCA6270900.071.529113
GACTCGC140150.071.329238
GCCACTT6427300.071.2703916
GTGCCAC6297350.071.2597314
CCACTTT6439600.071.21400517
TGCCACT6435900.071.0797415
CACTTTT6469100.071.0045518
ACTTTTT6454550.070.96306619
Produced by FastQC (version 0.11.5)