FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n02_CMV_S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n02_CMV_S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8992210
Sequences flagged as poor quality0
Sequence length80
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGT2714943.0192132968424894No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAAA1628871.8114234431802638No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTAA1551721.7256269593348021No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTTA1474441.639685905911895No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTATT1467831.6323350989356344No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTATA1460731.624439375859772No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAAT1453961.6169106370958863No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAA1401191.558226509389794No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTAT1340371.4905901886188158No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAAG1321191.4692606155772607No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTTT1317941.4656463761411265No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGC1311331.458295569164866No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTTG1287161.4314167484967544No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTATG1284121.428036044531878No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAGA1283551.4274021625384639No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGA1273861.4166261686504207No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAAC1235661.3741449543549362No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTT1234161.3724768438459511No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAT1223561.3606888629157905No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAG1220951.3577863506301566No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTAG1211791.3475997557886215No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGG1187951.3210879194324865No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGGA1166671.2974229916783528No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTAC1158031.287814675146599No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTA1139311.2669966559944663No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTACA1136561.2639384533946605No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCAA1134011.261102665529386No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTTC1118911.2443103530722703No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTA1113581.2383830003970102No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTG1104761.2285745106041785No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCAT1092861.2153408338995642No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTCT1058031.1766073078809325No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAGT1057331.1758288563100727No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAC1054501.1726816878164543No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTACT1051041.1688339129090624No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTC1050301.1680109783912964No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTACC1038221.154577128425604No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTT1029591.144979932630577No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAGG977771.0873522749135085No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTCC950801.057359647961958No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTATC950101.0565811963910985No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGCA938801.044014763890078No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCGT917181.019971731087241No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTACG916901.0196603504588972No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGGG892470.9924923906358949No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGTG885630.9848858067149233No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGCT877700.9760670624907559No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTCA873000.9708403162292696No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGGT867490.9647127902929313No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGCC851690.9471420262649559No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCAG845690.940469584229016No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCCT834430.927947634674902No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTCG819100.9108995452730753No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAGC812770.9038601189251585No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGGC809680.9004238112766495No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCGA798840.8883689326650512No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTC798220.8876794469880042No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCGG766550.8524600737749675No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCAC763710.8493017845446226No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCCA759320.8444197811216598No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCCG744900.8283836787619505No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCCTGT739420.8222895150357922No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCGC709490.7890051500131781No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCCC709000.7884602339135763No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGCG611120.6796104628339419No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG584610.6501293897718136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCG8227100.073.961861
GATCCGA8261150.073.662962
ATCCGAC8304350.073.351253
TCCGACT8325950.073.0558554
GACTCGG8355950.072.8428967
CGACTCG8348400.072.8416066
ACTCGGT8367150.072.6578458
CTCGGTG8367400.072.524929
TCGGTGC8364800.072.444410
CCGACTC8383350.072.4107365
CGGTGCC8342700.072.3161311
GGTGCCA8373950.072.156312
GTGCCAC8409300.071.9700113
GCCACTT8399150.071.940715
CCACTTT8441500.071.8501916
TGCCACT8413350.071.7352914
ACTTTTT8447750.071.658618
CACTTTT8470200.071.6124517
CTTTTTC8438050.071.51720419
TCCTCTT191500.071.4676633
Produced by FastQC (version 0.11.5)