FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n02_CMV_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n02_CMV_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9718959
Sequences flagged as poor quality0
Sequence length80
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGTCTC915070.9415308779469077No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG603370.6208175175962776No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCCTGTCTC412950.4248911843336308No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTGTCT220500.22687615000742362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACT9127000.073.751611
GACTCGG9147000.073.52014
CGACTCG9142900.073.515893
ACTCGGT9192950.073.3425145
CTCGGTG9183050.073.261616
TCGGTGC9156750.073.150217
CGGTGCC9120450.073.0757148
CCGACTC9222900.072.946852
GGTGCCA9164500.072.915499
GCCACTT9172900.072.77824412
GTGCCAC9184900.072.7712910
CCACTTT9184600.072.7118113
TGCCACT9196850.072.61159511
ACTTTTT9182000.072.4685715
CACTTTT9235450.072.4628814
CTTTTTC9154000.072.3596316
TTTTTCA9172900.072.16260517
CAACTAG9266400.071.74837522
AACTAGT9286650.071.7046723
TCAACTA9268300.071.7025921
Produced by FastQC (version 0.11.5)