FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29686441
Sequences flagged as poor quality0
Sequence length70
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9705173.2692265132085048No Hit
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA5347891.8014587871951375No Hit
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG727030.24490305186802286No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT603520.20329819933618853No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT460360.1550741633192069No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA354470.11940468040611536No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGAAGATCGGAAGAGCACACGTCTGAACTC350070.11792252227203659Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG345890.11651447204466174No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATAGATCGGAAGAGCACACGTCTGAACT323690.10903631054999151Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT299320.10082717561192331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACTC108900.034.82971664
AGCGAAC2388200.034.6918339
TAGCGAA2919500.028.326718
AAGGTAG2961750.028.1795674
GGTAGCG2939300.028.1655776
CTAAGGT2936700.028.1399172
AGGTAGC2967700.028.047695
CCTAAGG2940400.028.021861
GCGAACC2968200.027.78060310
GTAGCGA3041250.027.2883057
GAACCAG3021400.027.2769312
TAAGGTA3076400.027.1055053
ACCAGTA3062100.026.97763414
CGAACCA3086000.026.77603111
AACCAGT3135800.026.38693213
AGTAGGT3176700.025.7192917
GTCCTAA1673100.024.76201655
CAGTAGG3329000.024.63452116
ATAAGGT33600.024.6195072
TAGGTCC3309550.024.57447219
Produced by FastQC (version 0.11.5)