FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n01_TRE_S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n01_TRE_S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8163455
Sequences flagged as poor quality0
Sequence length70
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA3442974.217540244908559No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATAGATCGGAAGAGCACACGTCTGAACT1721552.1088497456040365Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG446880.5474152794374441No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT338700.41489785881100594No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATAGATCGGAAGAGCACACGTCTGAAC306370.3752945290933802Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT264870.3244582104023358No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA240880.29507114328430795No Hit
CTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAAC211300.2588364852871731No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG189490.23211985611484354No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT181200.2219648420919819No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATGA175780.21532549637377799No Hit
CTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGT159780.19572595181819463No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT134440.16468517312828942No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA130500.159858785281477No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT127190.1558041295015407No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT112880.1382747868396408No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA94780.1161028020611371No Hit
CATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT86630.10611928405313681No Hit
GCCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCC85220.10439207418917604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACT250950.050.2147864
TCTGAAC282300.044.92170363
GTCTGAA302300.041.82268562
CGTCTGA321400.039.34722561
ACGTCTG349150.036.21079360
AGCGAAC1254300.034.655489
CACGTCT373050.033.95093559
CTAGCCG1003.014793E-828.8037742
ACACGTC445350.028.46075458
GCGAACC1608050.026.96390510
CGAACCA1614250.026.84844811
ACCAGTA1627500.026.52762614
AACCAGT1634350.026.492813
TAGCGAA1647100.026.3714248
GAACCAG1642100.026.34438312
GGTAGCG1673750.025.9966166
GTAGCGA1681200.025.9126647
AGGTAGC1688750.025.852885
CTAAGGT1731400.025.2332922
TAAGGTA1738050.025.2097853
Produced by FastQC (version 0.11.5)