FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n01_TRE_S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n01_TRE_S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8183029
Sequences flagged as poor quality0
Sequence length70
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA3659744.472353696901233No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGAAGATCGGAAGAGCACACGTCTGAACTC1121351.3703360943728784Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG513210.627163853384853No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT453310.5539635751015913No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT335720.4102637299708946No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA257730.3149567232378133No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG214230.261797923482857No Hit
CTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGT185370.22652980943853432No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATGA178080.21762112782442786No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGAAGATCGGAAGAGCACACGTCTGAACT167850.20511964457171056Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT157930.19299699414483318No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT146510.17904128165744004No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA143530.1753995983638821No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT132020.16133390215285806No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT117820.14398091464664245No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA113320.13848172846509527No Hit
CTTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT102290.12500261211343622No Hit
CATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT87790.10728301219511747No Hit
AGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAG85590.10459452117302774No Hit
GATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT82680.10103838077562721No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACTC160050.050.4439564
CTGAACT183700.044.594263
TCTGAAC192550.042.49469862
GTCTGAA217550.037.59666461
AGCGAAC1319350.036.3544439
CGTCTGA226650.036.03067860
ACGTCTG253850.032.15739459
TAGCGAA1725850.027.7397448
GCGAACC1724050.027.68332510
CGAACCA1731800.027.53911611
GGTAGCG1753900.027.4505566
AGGTAGC1763900.027.3455665
GTAGCGA1762200.027.2946517
AACCAGT1752050.027.18595313
ACCAGTA1750300.027.16011614
GAACCAG1758850.027.07737412
TAAGGTA1817250.026.5691833
AAGGTAG1825250.026.535144
CTAAGGT1812350.026.509392
CCTAAGG1820200.026.4098241
Produced by FastQC (version 0.11.5)