HVV32AFXY_n01_TRE_S4.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HVV32AFXY_n01_TRE_S4.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10064908
Sequences flagged as poor quality	0
Sequence length	70
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA	396965	3.9440499605162813	No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGAAGATCGGAAGAGCACACGTCTGAACTC	175062	1.7393303545347856	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG	54162	0.5381271244605514	No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT	47317	0.47011855448653883	No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT	35369	0.35140907398259374	No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG	32822	0.32610332851527307	No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA	26885	0.267116202155052	No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATGA	24719	0.24559588622171213	No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGAAGATCGGAAGAGCACACGTCTGAACT	24423	0.24265497508770073	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT	21221	0.21084147018532112	No Hit
CTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGT	21101	0.20964920891477595	No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA	17883	0.17767673584299032	No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT	14379	0.14286270674307208	No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT	14181	0.1408954756466726	No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA	14167	0.1407563784984423	No Hit
CTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAAC	13413	0.13326500351518364	No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT	11681	0.11605669917698204	No Hit
GTTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT	11129	0.11057229733247437	No Hit
CATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT	10881	0.10810829070668107	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGAACTC	24285	51.810337	64
CTGAACT	28150	45.026424	63
TCTGAAC	29185	43.484455	62
GTCTGAA	33430	38.077778	61
AGCGAAC	161840	36.92441	9
CGTCTGA	35020	36.284985	60
ACGTCTG	38975	32.63579	59
GAACTCC	4495	30.112839	64
TAGCGAA	206365	28.940601	8
GGTAGCG	208605	28.730795	6
AGGTAGC	210155	28.560152	5
GTAGCGA	209985	28.528545	7
TAAGGTA	214975	27.888535	3
AAGGTAG	215690	27.868793	4
GCGAACC	213910	27.86147	10
CTAAGGT	214600	27.833143	2
CGAACCA	214515	27.7456	11
CCTAAGG	215530	27.743835	1
ACCAGTA	216885	27.368643	14
AACCAGT	217280	27.349815	13

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content