FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n01_TRE_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n01_TRE_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9505537
Sequences flagged as poor quality0
Sequence length70
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA4236424.4567918677293035No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGATCGTAGATCGGAAGAGCACACGT1491691.569285354420271No Hit
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG591530.6223004549874458No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT572190.6019544187771821No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT440750.4636771178735089No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT284420.299215078537909No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG278880.2933868965004292No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA269080.2830771160009161No Hit
CTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGT247350.26021675577087333No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATGA224920.2366199826480082No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGATCGTAGATCGGAAGAGCACACG222710.2342950219435262No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA183100.19262457239396363No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT172430.18139953587051422No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT172360.18132589458123197No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA166040.17467713817746436No Hit
GCCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCC148320.15603537180487542No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT127160.13377466207327374No Hit
CATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT102560.10789500898265927No Hit
TCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAG98150.10325560775787838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT204050.051.95471664
GCACACG231400.045.95228663
AGCACAC256950.041.44525562
AGCGAAC1808900.038.343089
GAGCACA292500.036.43032561
AGAGCAC324600.032.8671360
ACACGTC36500.031.2979264
TAGCGAA2285250.030.3534368
GGTAGCG2313100.030.0542246
GTAGCGA2326550.029.9095157
AGGTAGC2334900.029.881755
TAAGGTA2384650.029.2811533
AAGGTAG2394100.029.2591534
CTAAGGT2378000.029.2265262
CCTAAGG2378550.029.1843511
AAGAGCA371550.028.77424259
GCGAACC2444200.028.30488210
CGAACCA2455000.028.17384711
GAACCAG2478950.027.83452612
AACCAGT2478850.027.83421313
Produced by FastQC (version 0.11.5)