FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n01_TRE_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n01_TRE_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6929651
Sequences flagged as poor quality0
Sequence length70
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA3070364.4307570467834525No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGATCGAGATCGGAAGAGCACACGTC1600752.3100008932628784No Hit
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG433150.625067553907116No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT265460.38307845517761285No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGATCGAGATCGGAAGAGCACACGT261840.37785452687299836No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA218520.3153405561117003No Hit
CTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAAC214010.3088322918426917No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT204700.2953972718106583No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG156270.22550919230997346No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATGA153450.22143972329919645No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT148850.2148015823596311No Hit
CTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGT128000.18471348701399248No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA107030.15445222277427825No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT104650.15101770637511183No Hit
CTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGATCGAGATCGGAAGAGCACACGTCT89550.1292272872039299Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT87390.12611024711056879No Hit
CATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT86660.1250568030049421No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT85380.12320966813480216No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA72690.10489705758630558No Hit
GAGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGATCGAGATCGGAAGAGCACACG70800.1021696475046146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC216650.053.33469464
CACACGT249000.046.73959463
GCACACG265450.043.9757462
AGCACAC295850.039.5435561
GAGCACA325500.035.95133260
AGAGCAC343000.034.18238459
AGCGAAC981750.033.4095239
CACGTCT39500.028.35384664
AAGAGCA419900.028.00609258
GCGAACC1224250.026.69484110
CGAACCA1226400.026.59586111
ACCAGTA1236000.026.32178714
AACCAGT1242750.026.2483413
GAACCAG1255400.025.92541112
GTCCTAA908200.025.57621855
TCCTAAG910700.025.48492656
GGTCCTA933300.024.92266354
TAGCGAA1330300.024.6511298
AGTAGGT1324950.024.42426917
GGTAGCG1351500.024.3109236
Produced by FastQC (version 0.11.5)