FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n01_TRE_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n01_TRE_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8979763
Sequences flagged as poor quality0
Sequence length70
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA3690714.110030520850049No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGATCAGATCGGAAGAGCACACGTCT1706281.9001392353005309Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG558050.6214529269870486No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT441540.49170562742023366No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT348850.3884846404075475No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA244830.2726463938970327No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG237820.2648399517893735No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT232940.2594055099227006No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGATCAGATCGGAAGAGCACACGTC207610.2311976385122859No Hit
CTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGT198280.2208076092876839No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATGA185060.20608561718165613No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT144910.1613739694466324No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT134720.15002623120454292No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT130160.1449481461815863No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA126430.1407943617220187No Hit
GATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT112970.1258051019832038No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA108570.1209051953821053No Hit
CTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAAC97390.10845497815476868No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT229000.054.63665864
ACACGTC256350.048.94478263
CACACGT279850.044.94906262
GCACACG311400.040.3949761
AGCGAAC1503100.037.7602779
AGCACAC350650.035.84598560
ACGTCTG36050.033.9966764
GAGCACA384900.032.7477159
TAGCGAA1927450.029.3954588
GGTAGCG1950850.029.0857756
AGGTAGC1963500.029.0009165
GTAGCGA1960950.028.9536577
CATAAGG15350.028.3645921
GCGAACC2001150.028.26312810
TAAGGTA2027000.028.1366123
AAGGTAG2030500.028.118064
CGAACCA2013500.028.08341811
CTAAGGT2020650.028.0808982
CCTAAGG2021750.028.0089821
AGAGCAC453100.027.87506758
Produced by FastQC (version 0.11.5)