FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n01_CMV_S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n01_CMV_S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8904815
Sequences flagged as poor quality0
Sequence length70
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA3542973.978712640296289No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGATAGATCGGAAGAGCACACGTCTG2041242.2922879363580266Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG507560.5699837672090886No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT417330.46865656389268057No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT334310.3754261037427504No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGATAGATCGGAAGAGCACACGTCT249060.27969138044979036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG231300.25974711434207226No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA226190.2540086458842772No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT215390.24188037595390807No Hit
CTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGT200210.22483341877400034No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATGA184790.20751694448452887No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA169650.19051490682288177No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT160760.18053154388945758No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT160730.1804978542507621No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT132410.1486948353222386No Hit
CATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT115830.13007569500320892No Hit
GCCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCC115740.12997462608712254No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA112540.1263810646262724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG279350.053.677964
CACGTCT307000.048.9684763
ACACGTC329950.045.70789362
CACACGT362550.041.7305361
GCACACG391700.038.68216360
AGCGAAC1528350.038.1455279
CGTCTGA43300.035.76838764
AGCACAC425600.035.73637859
CATAAGG12700.030.5002941
TAGCGAA1921150.030.3312688
GGTAGCG1945350.030.033376
GTAGCGA1955750.029.8831867
AGGTAGC1965300.029.8262065
GAGCACA514150.029.58164458
TAAGGTA2016250.029.126643
CTAAGGT2007000.029.1208762
AAGGTAG2019750.029.1141994
CCTAAGG2013050.029.0222241
GCGAACC2049000.028.38092610
CGAACCA2058450.028.23975211
Produced by FastQC (version 0.11.5)