FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n01_CMV_S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n01_CMV_S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6894669
Sequences flagged as poor quality0
Sequence length70
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA2894024.197474889657502No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAAGATCGGAAGAGCACACGTCTGA1633732.3695553767700814Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG386500.5605780350006649No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT323440.4691160663405306No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAAGATCGGAAGAGCACACGTCTG281930.40891013042105423Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT241990.35098131614440087No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA201010.2915440900788711No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG168210.2439711028912338No Hit
CTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAAC162580.23580537368798998No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATGA152950.2218380606813757No Hit
CTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGT146530.2125265186769662No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT123480.17909489201004428No Hit
CTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAAGATCGGAAGAGCACACGTCTGAA110250.1599061535803967Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA104920.1521755431624056No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT102830.1491442156251446No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT94970.13774410345152177No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT89950.1304631157782919No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA81830.11868590065744998No Hit
GAGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAAGATCGGAAGAGCACACGTCT71480.10367430256622907Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAG70770.10264452144113081No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA231650.052.1325664
ACGTCTG269500.044.8464163
CACGTCT288900.042.03428662
ACACGTC312100.039.04323261
CACACGT337200.036.0895460
AGCGAAC1109700.034.3695379
GCACACG363550.033.59700859
GTCTGAA52500.032.97443864
AGCACAC437100.027.9949558
GCGAACC1423200.026.6952510
CGAACCA1429100.026.56264711
TAGCGAA1442800.026.408018
AACCAGT1442150.026.28678113
ACCAGTA1441100.026.2437614
GGTAGCG1462950.026.1370776
GAACCAG1450400.026.12843512
AGGTAGC1471450.026.0556965
GTAGCGA1470000.026.0086217
AAGGTAG1513900.025.3779454
TAAGGTA1509200.025.3657863
Produced by FastQC (version 0.11.5)