FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n01_CMV_S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n01_CMV_S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8992210
Sequences flagged as poor quality0
Sequence length70
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA3792404.217428196183141No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAGATCGGAAGAGCACACGTCTGAA2224292.473574349353496Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG512610.5700600853405337No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT410600.45661744999282716No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAGATCGGAAGAGCACACGTCTGA309120.34376421369162863Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT302030.3358796113524929No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA258880.2878936323773577No Hit
CTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAAC249180.2771065177525881No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG214920.2390068737273707No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT205030.22800846510479625No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATGA195740.21767730068581584No Hit
CTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGT182520.2029756867332947No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA134140.1491735624501652No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT132160.146971656578305No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT130470.14509225207151524No Hit
CTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAGATCGGAAGAGCACACGTCTGAAC112200.12477466607207793Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA108310.12044869948544353No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT102980.11452134681018349No Hit
GAGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCGAGATCGGAAGAGCACACGTCTG98890.10997296548901772Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT96020.10678131404849309No Hit
GTTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT90300.10042025264089696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGAA323250.050.3970964
CGTCTGA347250.046.9139263
ACGTCTG372800.043.7158262
CACGTCT401800.040.73605761
ACACGTC430900.038.08899360
CACACGT461250.035.62438659
AGCGAAC1425500.035.1801839
TCTGAAC61300.031.37291764
GCACACG545750.030.12615258
GCGAACC1818700.027.51446210
CGAACCA1824900.027.42624311
ACCAGTA1846300.027.09795414
AACCAGT1853900.027.04209913
GAACCAG1856950.026.96666712
TAGCGAA1867300.026.851478
GGTAGCG1886350.026.6542996
GTAGCGA1896250.026.5108667
AGGTAGC1902050.026.504965
AGCACAC632650.026.02852657
TAAGGTA1947900.025.8745083
Produced by FastQC (version 0.11.5)