FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n01_CMV_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n01_CMV_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8753825
Sequences flagged as poor quality0
Sequence length70
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA3477863.9729603916002434No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCAGATCGGAAGAGCACACGTCTGAAC1942432.2189500018563315Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG484370.5533238327245518No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT415060.4741470157331224No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT331960.3792170851027979No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCAGATCGGAAGAGCACACGTCTGAA251870.2877256513581206Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA236050.2696535514475101No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG228090.2605603836037389No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT224250.2561737297695579No Hit
CTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGT194610.22231424548697282No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATGA189430.21639683224190567No Hit
CTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAAC168370.19233877762006893No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA145520.166235902591153No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT139870.1597815811945064No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT133380.1523676792716327No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA129650.1481066847920766No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT106230.12135266583464942No Hit
GCCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCC101440.11588077211961628No Hit
GAGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATCAGATCGGAAGAGCACACGTCTGA99720.11391591675638936Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAAC263550.053.05962864
GTCTGAA296200.047.47017363
CGTCTGA316500.044.48614562
ACGTCTG341550.041.18596361
CACGTCT376700.037.5382660
AGCGAAC1446750.036.6297459
ACACGTC405700.034.9101959
CACACGT488500.029.02571958
TAGCGAA1839800.028.767748
GGTAGCG1862900.028.522986
GTAGCGA1874850.028.3753327
AGGTAGC1884750.028.2840045
GCGAACC1875700.028.17107410
CGAACCA1883550.028.01969111
TAAGGTA1928550.027.6434543
AACCAGT1909800.027.62618413
AAGGTAG1938450.027.6227994
ACCAGTA1907000.027.5996314
CTAAGGT1925950.027.5712572
GAACCAG1912200.027.54800212
Produced by FastQC (version 0.11.5)