FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n01_CMV_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n01_CMV_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10529484
Sequences flagged as poor quality0
Sequence length70
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA4158243.949139388026992No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATAGATCGGAAGAGCACACGTCTGAACT2599562.468838928859192Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG558150.5300829556320138No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT410260.3896297292440921No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT301120.28597792636372305No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG294920.28008969860251465No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATAGATCGGAAGAGCACACGTCTGAAC293420.2786651273699642Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA276520.26261495814989605No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT223370.21213765080985927No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATGA218740.20774047427205358No Hit
CTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGT196510.18662832860565626No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA170230.1616698406113728No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT147520.14010183215055932No Hit
CATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT132140.12549522844614228No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT131850.1252198113411825No Hit
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT124580.11831538943408812No Hit
CTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATAGATCGGAAGAGCACACGTCTGAACTC121490.11538077269503426Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA121070.11498189274992013No Hit
GAGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGATAGATCGGAAGAGCACACGTCTGAA119010.11302548159055087Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACT360950.051.5788164
TCTGAAC390250.047.91126363
GTCTGAA424000.044.14285362
CGTCTGA447400.041.85554561
ACGTCTG477250.039.2376560
AGCGAAC1529400.036.8764049
CACGTCT515100.036.44762459
TGAACTC70150.030.83640164
ACACGTC614600.030.5990458
TAGCGAA2004800.028.1222938
GGTAGCG2028250.027.8364876
AGGTAGC2047600.027.6828445
GTAGCGA2043350.027.657647
GCGAACC2058050.027.29825810
CGAACCA2063200.027.20220211
CTAAGGT2089800.027.0433062
TAAGGTA2099700.027.0416743
AAGGTAG2105550.027.0303824
CCTAAGG2096950.026.9300121
AACCAGT2091100.026.77805113
Produced by FastQC (version 0.11.5)