FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n01_CMV_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n01_CMV_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9718959
Sequences flagged as poor quality0
Sequence length70
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAA3996324.111880706565384No Hit
GCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGAAGATCGGAAGAGCACACGTCTGAACTC1275541.3124245096619918Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAG582500.5993440243960284No Hit
CCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT483360.4973372148189945No Hit
GCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT387060.398252528897385No Hit
GTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGG303020.31178236269954424No Hit
AGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGA264920.27258063337853367No Hit
ACTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT255060.2624355139269545No Hit
GTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGTAGGTCCACTATGA232590.23931575387857898No Hit
CTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTAAGGTAGCGAACCAGT216540.22280163955831073No Hit
AGCTGTGTAAGCGGAACTAGTTGAAAAAGTGGCACCGAGTCGGAAGATCGGAAGAGCACACGTCTGAACT199440.2052071626189595Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT154830.15930718505963448No Hit
GTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA151690.15607638637018634No Hit
TCTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT144030.14819488383478108No Hit
AGTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT134390.13827612607481934No Hit
CTAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCTA132510.13634176252827077No Hit
GATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT119030.12247196433280559No Hit
CATAGAACCCAGCTTTCTTGTACAAAGTGGTTTAATGAGTTTAAACCTCGAGCGTAACTATAACGGTCCT98060.10089557945454858No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACTC185150.049.87869664
CTGAACT209500.044.3715563
TCTGAAC218700.042.53424562
AGCGAAC1583350.037.7869579
GTCTGAA253150.036.7965261
CGTCTGA263100.035.34412860
ACGTCTG294450.031.54844559
TAGCGAA2037100.029.3104828
GGTAGCG2060100.029.0278116
AGGTAGC2080350.028.9140365
GTAGCGA2072100.028.874087
TAAGGTA2140000.028.1768863
CTAAGGT2129700.028.147082
AAGGTAG2144650.028.1381784
CCTAAGG2133400.028.1166291
GCGAACC2149300.027.71785410
CGAACCA2164150.027.5572311
CATAAGG16800.027.2482781
AACCAGT2193800.027.2120813
ACCAGTA2192550.027.16629614
Produced by FastQC (version 0.11.5)