FastQCFastQC Report
Tue 15 Nov 2022
HVV2HAFX3_n01_nov2022_11644-Spk-in.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2HAFX3_n01_nov2022_11644-Spk-in.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850743
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTGG43010.5055580827582478TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTGG31180.3665031625296946TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGGG17460.20523236747172763TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTTG14250.1675006435551042TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATGG13110.15410059207069587TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTTG13070.1536304148256289TruSeq Adapter, Index 8 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)