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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

         Loading report..

        Report generated on 2018-04-25, 02:04 based on data in: /mnt/gencore/sites/core-fastqc.bio.nyu.edu/html/HVV2GBGX5/merged

        General Statistics

        Showing 24/24 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HVV2GBGX5_n01_akm1
        31.1%
        41%
        11.9
        HVV2GBGX5_n01_akm10
        34.0%
        42%
        12.9
        HVV2GBGX5_n01_akm11
        30.0%
        45%
        10.7
        HVV2GBGX5_n01_akm12
        12.3%
        41%
        15.1
        HVV2GBGX5_n01_akm2
        15.6%
        40%
        11.5
        HVV2GBGX5_n01_akm3
        11.3%
        40%
        9.9
        HVV2GBGX5_n01_akm4
        14.2%
        41%
        6.8
        HVV2GBGX5_n01_akm5
        12.1%
        39%
        11.0
        HVV2GBGX5_n01_akm6
        10.0%
        40%
        11.7
        HVV2GBGX5_n01_akm7
        32.2%
        39%
        72.0
        HVV2GBGX5_n01_akm8
        29.8%
        40%
        16.4
        HVV2GBGX5_n01_akm9
        26.4%
        42%
        18.4
        HVV2GBGX5_n01_las71
        31.6%
        47%
        14.0
        HVV2GBGX5_n01_las72
        11.7%
        42%
        11.4
        HVV2GBGX5_n01_las73
        32.2%
        48%
        14.7
        HVV2GBGX5_n01_las74
        28.8%
        48%
        11.1
        HVV2GBGX5_n01_las75
        33.5%
        49%
        12.3
        HVV2GBGX5_n01_las76
        12.8%
        42%
        8.6
        HVV2GBGX5_n01_las77
        9.6%
        40%
        11.3
        HVV2GBGX5_n01_las78
        8.9%
        42%
        7.1
        HVV2GBGX5_n01_las79
        7.2%
        38%
        7.4
        HVV2GBGX5_n01_las80
        10.4%
        39%
        12.5
        HVV2GBGX5_n01_las81
        25.5%
        41%
        15.3
        HVV2GBGX5_n01_las82
        25.0%
        40%
        10.6

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Number of LanesTotal # of Single-End ReadsTotal # PF ReadsUndetermined % PhiX Aligned
        4.0
        368633600
        355676911
        3.1
        0.9

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.
        Perfect: The percentage of reads in this library which have a barcode perfectly matching the barcode specified in TuboWeb. The remainder of reads have mismatches upto the max number of mismatches specified in TuboWeb (Allowed barcode mismatch option in your Library Pool).

        Showing 24/24 rows and 3/3 columns.
        LibraryTotal Read CountPortion (%)Perfect (%)
        las80
        12511327
        3.6
        99.0
        akm6
        11669487
        3.4
        98.4
        akm2
        11456007
        3.3
        99.0
        las75
        12315932
        3.6
        96.9
        las82
        10638552
        3.1
        98.8
        akm5
        10997362
        3.2
        98.6
        las79
        7358850
        2.1
        99.0
        akm1
        11919784
        3.5
        98.8
        las71
        13984833
        4.1
        98.8
        las78
        7084482
        2.1
        98.8
        akm3
        9949032
        2.9
        99.1
        akm7
        72027848
        20.9
        98.4
        akm4
        6835838
        2.0
        97.2
        las77
        11313276
        3.3
        98.2
        akm9
        18390037
        5.3
        99.1
        las76
        8628759
        2.5
        98.9
        akm11
        10685864
        3.1
        98.6
        akm8
        16404299
        4.8
        98.9
        las81
        15276123
        4.4
        99.0
        akm10
        12853905
        3.7
        99.0
        las72
        11435544
        3.3
        98.8
        las74
        11077421
        3.2
        98.2
        las73
        14683017
        4.3
        98.4
        akm12
        15133823
        4.4
        98.8

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

        loading..

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

        loading..

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        loading..

        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (75bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        loading..

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        loading..

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        loading..

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.