FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_las82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_las82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10638552
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG7668587.208293008296618TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG3455343.2479420131611896TruSeq Adapter, Index 27 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG711300.668606028339195No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTGTGCGTGAAAAAAAAAG339820.3194231696193241TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCGTCTGCGTGAAAAAAAAAG287130.2698957527302588TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA155130.14581871668249588TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGGCGTCTTCTGCGTGAAAAAAAAAG134890.12679357115517226TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCGGCGTGAAAAAAAAAG132660.1246974212280017TruSeq Adapter, Index 27 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCGTGTGCGTGAAAAAAAAAG107760.10129198033717371TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTGA598150.066.9311560
CTCGTAT1714650.066.9282644
TCGTATG1704100.066.8713845
CGTATGC1582350.066.8646546
GTATGCC1591000.066.799147
TATGCCG1572950.066.7919148
CCGTCTT1349300.066.7642452
GCGTGAA710750.066.69906661
ATGCCGT1523650.066.673149
CCTTTAT1978350.066.6725337
CTGCGTG493300.066.64017559
TCCTTTA1983400.066.5731936
TGCCGTC1496800.066.54520450
CAGTCAC2010350.066.53921527
TCCAGTC2009100.066.4904625
CCAGTCA2010200.066.4687326
TATCTCG1844900.066.4660741
TCTCGTA1730050.066.4241443
GCCGTCT1355400.066.42142551
ACTCCAG2013350.066.3914923
Produced by FastQC (version 0.11.2)