FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_las81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_las81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15276123
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG184086512.0506034155394TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG2082211.3630487264340565TruSeq Adapter, Index 25 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG846860.5543684087906336No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA282000.1846018129076337TruSeq Adapter, Index 25 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCGGCGTGAAAAAAAAAG202620.1326383664232083TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT2362450.066.6999552
CGTATGC2439700.066.55163646
TATGCCG2438750.066.4350448
GTATGCC2443450.066.43347
CTCGTAT2449150.066.3569844
ATGCCGT2436850.066.3425949
TCGTATG2453750.066.3297145
GCCGTCT2376650.066.329651
TGCCGTC2428500.066.2443150
GTCACAC2482450.066.2328529
CACACTG2482000.066.094231
CTTCTGC2314250.066.08758556
CAGTCAC2498800.066.0385327
TATCTCG2471750.065.9689441
CCAGTCA2499000.065.9339426
CGTCTTC2367200.065.9173553
TCTCGTA2466050.065.9148143
ACTCCAG2500550.065.88393423
TCCAGTC2501650.065.8501925
AGTCACA2505650.065.81122628
Produced by FastQC (version 0.11.2)