FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_las80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_las80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12511327
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG2078691.6614464636724786TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG896520.7165666759409294No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG455340.3639422101268714TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC341750.064.138146
TATGCCG343800.063.5112748
GTATGCC345800.063.50296447
ATGCCGT342300.063.0848349
TGCCGTC338050.062.9298750
CGAGTGG364800.062.91280733
TCGTATG354250.062.76050645
GCCGTCT322150.062.7305451
CTCGTAT354900.062.6941244
ACGAGTG366950.062.66454332
AGGGGGG258250.062.046351
CACGAGT372050.061.90744431
AGTCACG373750.061.7491928
CCGTCTT329550.061.302352
GTCACGA376700.061.22902329
GTGGATA376150.060.77623736
GGATATC377050.060.3528438
TCACGAG382900.060.30228830
TATCTCG375800.060.1150841
TCTCGTA371600.059.9322443
Produced by FastQC (version 0.11.2)