FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_las79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_las79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7358850
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG605690.8230769753426147TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG293670.3990705069406225TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG191450.064.8815933
CGTACGT191800.064.7627634
GTACGTA195600.063.66316635
TACGTAA198650.062.77245736
CGTATGC143850.061.97838646
GTCACCG202600.061.97263729
CACCGTA201300.061.9615331
CGTAATC199750.061.90535738
CTCGTAT161800.061.70631444
ACGTAAT202750.061.4143237
GTATGCC145850.061.3394947
TATGCCG143200.061.2476848
TCGTATG160350.061.12519545
AGTCACC206050.061.06881328
ACCGTAC203900.061.03621332
ATGCCGT137500.060.6286749
TGCCGTC135600.060.43618450
TCACCGT207900.060.31316430
GTAATCT192650.060.09100739
CAGTCAC214500.059.01696427
Produced by FastQC (version 0.11.2)