FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_las78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_las78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7084482
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72450.10226576904281781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT93750.050.32965529
AGTCACG96250.049.38048628
TCGGGGG28600.047.84107643
CGTTTCG98900.047.0812333
CGGAATC97700.047.05613738
GTTTCGG100400.046.24051334
CGGGGGT25300.045.90773444
ATCGGGG32150.045.88167642
CAGTCAC106050.044.78476727
CCAGTCA106850.044.51397326
ACTCCAG109050.044.18047723
ACGTTTC107100.043.79707732
TCCAGTC109400.043.50667625
GAACTCC113150.043.15828321
TCACGTT110750.042.729830
TCGGAAT110300.042.14940637
CACGTTT112400.041.85456531
CGTCTGA118750.041.239716
ACGTCTG119800.040.93578315
GGGTGGC32400.040.845847
Produced by FastQC (version 0.11.2)