FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_las77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_las77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11313276
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG1328791.1745404248954945TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG623960.5515290177663835TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA381350.064.77608536
GCCTTAT389550.063.2682337
CGTATGC295150.063.06073846
CTCGTAT326750.062.7619644
TCGTATG324150.062.6701345
GTATGCC301400.062.0486547
CGTGGCC399350.061.91763733
ATGCCGT287250.061.79474349
TATGCCG299600.061.5819448
CTTATCT372000.061.27050439
TGGCCTT403500.061.2629235
CCTTATC401750.061.0816338
TGCCGTC285850.060.97673450
GTGGCCT404950.060.9499634
TATCTCG368800.060.6318841
GCCGTCT261900.060.55377251
TCTCGTA344150.059.68860243
GTCACGT421450.059.35045629
TTATCTC377100.059.3247840
AGTCACG423300.059.13991528
Produced by FastQC (version 0.11.2)