FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_las76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_las76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8628759
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG1812102.100070241850537TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG195080.2260811780697549TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT261850.063.76841744
TCGTATG262800.063.52404845
CGTATGC263500.063.39380346
GTATGCC269200.062.05150647
CACGTGA272250.062.01819231
TCACGTG272550.061.97916430
TCTCGTA268800.061.85039543
AGTCACG274950.061.77447528
GTCACGT274300.061.70689829
CCGTCTT263400.061.3800752
GCCGTCT263900.061.32577551
GATCTCG270800.061.07578341
ATCTCGT273500.060.86347642
TATGCCG275650.060.4620248
ACGATCT270100.060.34438739
ATGCCGT277650.059.95202349
CAGTCAC283450.059.77609327
CGATCTC273500.059.62856340
CTGCTTG238350.059.41147259
AACGATC274800.059.38651338