Basic Statistics
Measure | Value |
---|---|
Filename | HVV2GBGX5_n01_las76.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8628759 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG | 181210 | 2.100070241850537 | TruSeq Adapter, Index 19 (97% over 40bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG | 19508 | 0.2260811780697549 | TruSeq Adapter, Index 19 (97% over 40bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 26185 | 0.0 | 63.768417 | 44 |
TCGTATG | 26280 | 0.0 | 63.524048 | 45 |
CGTATGC | 26350 | 0.0 | 63.393803 | 46 |
GTATGCC | 26920 | 0.0 | 62.051506 | 47 |
CACGTGA | 27225 | 0.0 | 62.018192 | 31 |
TCACGTG | 27255 | 0.0 | 61.979164 | 30 |
TCTCGTA | 26880 | 0.0 | 61.850395 | 43 |
AGTCACG | 27495 | 0.0 | 61.774475 | 28 |
GTCACGT | 27430 | 0.0 | 61.706898 | 29 |
CCGTCTT | 26340 | 0.0 | 61.38007 | 52 |
GCCGTCT | 26390 | 0.0 | 61.325775 | 51 |
GATCTCG | 27080 | 0.0 | 61.075783 | 41 |
ATCTCGT | 27350 | 0.0 | 60.863476 | 42 |
TATGCCG | 27565 | 0.0 | 60.46202 | 48 |
ACGATCT | 27010 | 0.0 | 60.344387 | 39 |
ATGCCGT | 27765 | 0.0 | 59.952023 | 49 |
CAGTCAC | 28345 | 0.0 | 59.776093 | 27 |
CGATCTC | 27350 | 0.0 | 59.628563 | 40 |
CTGCTTG | 23835 | 0.0 | 59.411472 | 59 |
AACGATC | 27480 | 0.0 | 59.386513 | 38 |