FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_las75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_las75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12315932
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG3998443.246559009906843TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG1027210.8340497495439241TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT728250.066.2720744
TCTCGTA732400.065.8304243
TCGTATG732200.065.8298545
ATCTCGT750900.065.2963242
ACATCTC755850.064.63621540
CACATCT755250.064.5337639
CGTATGC734000.064.4192146
CGCACAT763050.064.4111937
CACGTCC777900.064.40880631
CATCTCG758050.064.3486341
GTATGCC735900.064.2965847
GTCCGCA770650.064.2798834
GTCTTCT621550.064.22626554
TCACGTC790750.063.8989430
GTCACGT793900.063.87415729
CCGTCTT658100.063.8621452
ACGTCCG784150.063.7501732
CCGCACA771050.063.6881636
AGTCACG797800.063.6226428
CGTCCGC784700.063.304633
Produced by FastQC (version 0.11.2)