FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_las74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_las74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11077421
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG1979411.786887038056963TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG339990.3069216201135625TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA313100.064.8555736
CTCGTAT313650.064.5165844
TCGTATG318200.063.8331345
TCTCGTA314800.063.7990243
CCGTCCC322050.063.79174834
CGTCCCG324650.063.17472535
CCCGTCC327850.062.86318633
ATCTCGT322200.062.237542
CCCGATC325250.061.58166538
CGTATGC329550.061.3102646
ACCCGTC339250.060.8789632
CACCCGT340850.060.805231
GTATGCC333050.060.75769847
GTCACCC342750.060.71460729
AGTCACC345550.060.65117328
CCGTCTT312400.060.12195652
GTCTTCT302800.059.918754
CCGATCT332700.059.83012439
CAGTCAC353250.059.76809327
GATCTCG335200.059.71068241
Produced by FastQC (version 0.11.2)