FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_las73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_las73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14683017
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG937510.6384995672210964TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGGCGTCTTCTGCGTGAAAAAAAAAG555450.37829418844914503TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGGCGTCTTGTGCGTGAAAAAAAAAG436230.297098341573806TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGTGGCGGGGTGGGGGGGGAAAAAAAAG161410.10992972357111622TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACATG2870800.066.4574930
AGTCACA2881900.066.42831428
CATGTCA2852500.066.3881333
GAACTCC2859200.066.3634321
CACATGT2873050.066.3566831
ACATGTC2858950.066.3449732
GTCACAT2880100.066.3233729
ACACGTC2895550.066.2705713
CACGTCT2894600.066.20376614
TGTCAGA2854650.066.2036635
CACACGT2895050.066.18349512
AGAATCT1826950.066.17827639
CAGTCAC2881600.066.1651327
ATGTCAG2859250.066.1224734
TCCAGTC2868150.066.0888225
GAATCTC1547300.066.0547940
AATCTCG1545300.066.0020141
GCACACG2904950.065.9683911
ACTCCAG2865050.065.9596923
AACTCCA2872000.065.94623622
Produced by FastQC (version 0.11.2)