FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_las72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_las72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11435544
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG348510.3047603157313723TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG260620.22790345610143253TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT155150.057.02245739
CTCGTAT130400.055.58045244
CCGTATC185350.055.37302438
GTTCCGT188450.054.7583435
GTCACAG190700.054.7096529
TCCGTAT190200.054.19683537
TCGTATG132900.054.0419845
AGTTCCG191500.053.958234
GTATCTC155400.053.44644540
CGTATGC116850.053.35370346
ACACGTC202500.053.0654113
CACACGT203350.052.9111512
TTCCGTA195600.052.82740836
CAGTCAC203800.052.09000827
GCACACG206450.052.0837211
ACGTCTG205500.051.9546415
CGTCTGA205700.051.8864616
GTATGCC121650.051.8708547
GAACTCC204650.051.84933521
AGTCACA204750.051.79759628
Produced by FastQC (version 0.11.2)