FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_las71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_las71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13984833
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG4828603.4527405511385085TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG1258150.8996532171674843TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT853750.066.0705444
TCGTATG860500.065.69668645
TCTCGTA860750.065.5530143
CACACGT912650.065.47874512
CACAGTC898500.065.4615531
ACACGTC912350.065.4598913
AGTCAAC894450.065.2051934
CACGTCT915500.065.1024414
GTCACAG904850.065.0834529
AGTCACA912100.064.9477928
GCACACG921950.064.866611
ATCTCGT889350.064.8322642
ACAGTCA905850.064.81602532
GAACTCC917200.064.6795621
TCACAGT912200.064.63222530
CGTATGC856350.064.50941546
GTATGCC858600.064.36661547
AGCACAC931850.064.2854110
AATCTCG896150.064.2287941
CAGTCAA911750.064.22523533
Produced by FastQC (version 0.11.2)