FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_akm9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_akm9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18390037
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG14750908.021136662204649TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2812691.529464024460636TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAG711790.38705196732339364TruSeq Adapter, Index 9 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG193870.10542121258374847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2211950.067.2228942
CGTATGC2230400.067.2227244
GTATGCC2232700.067.1829845
TCGTATG2237050.067.0446443
TATGCCG2243400.066.8865846
CTGCTTG2003250.066.8735657
GCCGTCT2167150.066.8237549
ATGCCGT2239050.066.8175147
TGCCGTC2207950.066.6159648
TCTCGTA2229750.066.6089641
CCGTCTT2175050.066.5937250
GATCTCG2232150.066.5107339
CAGATCT2232200.066.4898137
AGATCTC2234150.066.4290538
AGTCACG2274950.066.4127328
GTCACGA2276400.066.30344429
CACGATC2275550.066.2627631
TGCTTGA2024300.066.2211758
ATCTCGT2246950.066.0912440
CTTCTGC2138250.066.08964554
Produced by FastQC (version 0.11.2)