FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_akm8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_akm8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16404299
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTGGCGGGGTGGGGGTGGAAAAAAAAAAG396560.24174150934459315TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGGTGGCGGGGTGGGGGTGGAAAAAAAAAAG276050.16827905904421764TruSeq Adapter, Index 8 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGGTGGCGGGGTGTGGGTGGAAAAAAAAAAG265820.16204288887931145TruSeq Adapter, Index 8 (97% over 45bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG251640.1533988133232636No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTGGCGGGGTGTGGGTGGAAAAAAAAAAG193420.11790811664673997TruSeq Adapter, Index 8 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC624850.065.695344
GTCACAC1518050.065.3882929
GTATGCC645750.065.2875545
CTCGTAT573850.065.17982542
CAGTCAC1536200.064.8145627
ACACTTG1522650.064.8023832
TATGCCG637950.064.71303646
CCAGTCA1537300.064.70969426
GCGGGGT411200.064.6086850
TCCAGTC1539850.064.552325
AGTCACA1542900.064.52988428
TCGTATG565700.064.47397643
TCACACT1536850.064.4386630
CACTTGA1529050.064.43610433
GAACTCC1550350.064.27740521
CGGGGTG424700.064.2754451
ACTCCAG1547100.064.256723
CTCCAGT1550850.064.0807224
CACACTT1544050.064.0203231
CGTCTGA1566850.063.88186616
Produced by FastQC (version 0.11.2)