FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_akm7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_akm7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72027848
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG7128450.989679713879554TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGGGTGGCGGGGTGGGGGTGGAAAAAAAAAAG792690.11005326717521813TruSeq Adapter, Index 7 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTGGCGGGGTGGGGGTGGAAAAAAAAAAG741360.10292685684570224TruSeq Adapter, Index 7 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2082150.065.0986744
AGTCACC4130750.064.8143828
GTATGCC2123450.064.4607745
TATGCCG2106250.064.1443246
CAGTCAC4202200.063.8953827
GCGGGGT1050400.063.84628350
CCAGTCA4189850.063.79813826
CTCGTAT2027250.063.70480742
GTCACCA4204600.063.65120329
ACCAGAT4196550.063.56331632
ACGTCTG4231650.063.55512215
TCGTATG2012850.063.49609843
ACTCCAG4201500.063.44256223
TCCAGTC4216050.063.38236225
CCAGATC4183250.063.37438233
CGGGGTG1102300.063.27584551
CGTCTGA4255700.063.2062616
CACGTCT4258750.063.20414714
ACACGTC4266050.063.18448613
GAACTCC4233000.063.05756421
Produced by FastQC (version 0.11.2)