FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_akm6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_akm6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11669487
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1485671.2731236600203593TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG216540.18556085627414468TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAG192600.1650458156386823TruSeq Adapter, Index 6 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC284650.065.1015444
TATGCCG287100.064.5091546
CTCGTAT293200.064.0729442
TCGTATG292650.064.0520343
ATGCCGT282550.063.70453347
GTATGCC291950.063.6977345
TGCCGTC256050.063.08350448
GCCGTCT244400.063.03067849
TATCTCG316550.062.2546339
TCTCGTA304750.061.8142141
CCGTCTT252800.060.93630650
ATCTCGT323700.060.74007840
CGCCAAT330100.060.2968933
ACGCCAA331500.060.24837532
GTCACGC335000.059.99236729
CACGCCA337750.059.21537831
GCCAATA338000.058.8875834
AGTCACG344600.058.7311528
CGTCTTC259800.057.06617751
TCACGCC352800.056.80116730
Produced by FastQC (version 0.11.2)