FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_akm5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_akm5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10997362
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1037030.9429806893689595TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAG244310.22215327639482996TruSeq Adapter, Index 5 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC344100.064.8417644
TATGCCG343100.064.437846
GTATGCC348600.064.13296545
TCGTATG318550.063.58355743
ATGCCGT286200.063.21798347
CACAGTG543050.062.6036633
GTCACAC549150.062.0594529
ACACAGT550150.061.9253232
CTCGTAT215400.061.88786742
TGCCGTC258600.061.7556248
CAGTGAT552850.061.3630135
CGGGGTG109850.061.3332151
GCCGTCT249400.061.3253249
CAGTCAC557550.061.30401227
CACACAG556300.061.29056531
ACAGTGA554100.061.26812434
CCAGTCA559950.060.7827426
AGTCACA563650.060.7017128
GCGGGGT87100.060.69545750
TCCAGTC563150.060.39286425
Produced by FastQC (version 0.11.2)