FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_akm4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_akm4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6835838
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG2569283.7585443072231968TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG337390.4935605554139814TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAG284630.4163790891475193TruSeq Adapter, Index 4 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC468150.065.1796644
TCGTATG474150.065.1030243
CTCGTAT468850.065.03039642
GTATGCC470050.064.9107545
GCCGTCT405000.064.4077149
TCTCGTA473400.064.3398541
TATGCCG474500.064.28746
ATGCCGT464850.064.16653447
TGCCGTC428850.063.7750248
ATCTCGT492300.063.71788440
ACTGACC506600.063.6110532
CCGTCTT410450.063.59447550
CCAATCT492900.063.58531637
GACCAAT503050.063.3908335
CTGCTTG356900.063.23750757
AATCTCG499550.063.11831339
GTCACTG517400.063.02219829
ACCAATC499100.062.98095736
CACTGAC515450.062.92675831
CTGACCA511450.062.8214433
Produced by FastQC (version 0.11.2)