FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_akm3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_akm3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9949032
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1028231.0334975302119844TruSeq Adapter, Index 3 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG295240.29675248808125254No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAG288080.28955580804243064TruSeq Adapter, Index 3 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG412500.064.3507332
CACTTAG414900.064.0947831
CGTATGC254300.063.819644
AGGCATC398600.063.70942736
TATGCCG252450.063.39958246
GTATGCC256650.063.38294645
CTCGTAT252050.062.3112242
TCGTATG248050.062.25975443
TTAGGCA425050.062.19405734
GGCATCT286800.062.17639537
ATGCCGT221750.062.05656447
TCACTTA437300.060.87866230
CAGTCAC438100.060.85975627
CCAGTCA439000.060.6486326
TAGGCAT434900.060.6107735
TGCCGTC200400.060.58495748
GCCGTCT194200.060.5316349
TCCAGTC442650.060.17937525
ACTCCAG442450.060.07771323
GCATCTC294600.059.80484838
Produced by FastQC (version 0.11.2)