FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_akm2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_akm2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11456007
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAG1064740.9294163315368085TruSeq Adapter, Index 2 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG292110.2549841319056457No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTGGCGGGGTGGGGGTGGAAAAAAAAAAG192910.16839200604538737TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGGTGGCGGGGTGGGGGTGGAAAAAAAAAAG173980.15186792396338444TruSeq Adapter, Index 2 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC868750.066.8555644
GTATGCC880400.066.569845
TATGCCG869350.066.54777546
CTCGTAT844150.066.4277842
TCGTATG833100.066.4070543
CGATGTA1420950.066.1218534
GTATCTC975650.066.0712638
CCGATGT1427050.065.85853633
TATCTCG978250.065.8143539
ATGTATC1422000.065.7751736
ATGCCGT714900.065.7185547
GATGTAT1431450.065.6970335
GTCACCG1439450.065.6917629
AGTCACC1447400.065.4553428
GCCGTCT612050.065.4216949
ACCGATG1440800.065.3979932
GCGGGGT257500.065.3907350
TCTCGTA860300.065.3530241
TGTATCT1016350.065.3108237
TGCCGTC636850.065.2171648
Produced by FastQC (version 0.11.2)