FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_akm1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_akm1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11919784
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG11656159.778826529071333TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2269991.9043885358996437TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAG871300.7309696215971699TruSeq Adapter, Index 1 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG257120.21570860680025744No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG151370.12699055620470975TruSeq Adapter, Index 1 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1909350.067.0290844
GTATGCC1911200.066.9629745
TATGCCG1910050.066.95998446
CTGCTTG1596150.066.9091757
CTCGTAT1902250.066.8879442
TCGTATG1917400.066.8339543
ATGCCGT1904950.066.7085247
CACGATC1906600.066.6284536
ACGATCT1905400.066.6151637
GATCTCG1910550.066.6105739
TGCTTGA1606700.066.51968458
CGATCTC1909400.066.4900438
TGCCGTC1838450.066.47089448
GCCGTCT1789650.066.44057549
TCTCGTA1910700.066.4388241
CCGTCTT1791150.066.41186550
TCACGAT1932350.066.3024335
CTTCTGC1734400.066.2280854
ATCTCGT1923400.066.1255540
ATCACGA1942700.066.12305534
Produced by FastQC (version 0.11.2)