FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_akm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_akm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15133823
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG801570.5296546682222991TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAG165340.1092519715606559TruSeq Adapter, Index 12 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT196550.058.8274742
CGTATGC202100.058.2010344
CCTTGTA276300.057.99275633
TCGTATG209250.057.5463943
GTATGCC205900.057.22751245
GTCACCT283550.056.72932429
CACCTTG283300.056.5925931
AGTCACC286600.056.46252428
ACCTTGT286850.055.9883232
TGTAATC253750.055.4177936
TATGCCG214400.054.71758346
GTAATCT237650.054.36970537
CTTGTAA294700.054.26646834
CAGTCAC304050.053.53943627
TAATCTC244950.052.83397338
ATGCCGT199600.052.8170247
TCTCGTA219800.052.79297341
GCCGTCT164100.052.73393249
TCCAGTC311700.052.1141625
CCAGTCA311150.052.10710526
Produced by FastQC (version 0.11.2)