FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_akm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_akm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10685864
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG4697984.396443750360289TruSeq Adapter, Index 11 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG652140.6102828933626706TruSeq Adapter, Index 11 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAAG549000.5137628553011717TruSeq Adapter, Index 11 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG254400.2380715307625102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT864700.066.1021842
ATGCCGT836650.066.0588247
CGTATGC862500.065.9910844
GTATGCC864950.065.8758845
TATGCCG866150.065.5856646
TCGTATG873950.065.4262143
GGCTACA919700.065.1434534
ACGGCTA928600.064.946632
TACATCT904050.064.93284637
CGGCTAC925400.064.83903533
TGCCGTC775300.064.7105848
CACGGCT936600.064.50960531
GTCACGG940400.064.41350629
CTACATC925400.064.2654736
GCTACAT931550.064.2000835
TCTCGTA892000.064.0443241
AGTCACG947900.064.0423228
ACATCTC917300.063.98740838
TCACGGC945500.063.9408730
GCCGTCT750700.063.86553649
Produced by FastQC (version 0.11.2)