FastQCFastQC Report
Wed 25 Apr 2018
HVV2GBGX5_n01_akm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV2GBGX5_n01_akm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12853905
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1710571.3307784677107852TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTGGCGGGGTGGGGGTGGAAAAAAAAAAG299930.23333765108735438TruSeq Adapter, Index 10 (97% over 42bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG253830.19747306363319161No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218250.16979275947659486No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGGTGGCGGGGTGGGGGTGGAAAAAAAAAAG167760.13051286749046304TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGGTGGCGGGGTGTGGGTGGAAAAAAAAAAG147970.1151167680171901TruSeq Adapter, Index 10 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTGGCGGGGTGTGGGTGGAAAAAAAAAAG131880.10259917122462006TruSeq Adapter, Index 10 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC539250.066.28210444
CTCGTAT490500.066.1034542
GTATGCC558550.065.6271845
CTTATCT615500.065.4403937
ATGCCGT391050.065.3473247
TAGCTTA1116050.065.2174334
TATGCCG552450.065.1852446
TTATCTC586650.065.1689638
ACTAGCT1120600.065.1392732
TCGTATG489700.065.0572543
GCTTATC1107500.065.0455936
AGCTTAT1122400.064.7839735
CTAGCTT1124950.064.72903433
GGGTGGA323450.064.68400659
TATCGCG299500.064.6368539
CACTAGC1132950.064.5934731
TCACTAG1139000.064.3907630
GCGGGGT276150.064.3572750
TGCGTGA180750.063.79318658
TGGGTGG218750.063.72809658
Produced by FastQC (version 0.11.2)