FastQCFastQC Report
Wed 3 Aug 2022
HVV25AFX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV25AFX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134176943
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAGCC3648307527.190271431359115No Hit
AAAGCATA3575889826.65055351574078No Hit
TGTAGCGG3185964223.74449833754224No Hit
GCCTTTAT1808092813.475435939839532No Hit
GGGGGGGG68824655.129394697865489No Hit
CTGCAGCA2709750.20195347571750835No Hit
TGCAGCCA1987150.14809921552617278No Hit
CTCAGCCA1790640.13345362921258386No Hit
TGTAGCGA1548010.11537079064321804No Hit
GCCTTATA1411900.105226722895304No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)