FastQCFastQC Report
Fri 9 Sep 2022
HVTWYAFX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTWYAFX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163728925
Sequences flagged as poor quality0
Sequence length6
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTT3736293722.819997749328653No Hit
GATCAG3671400522.423652387627904No Hit
ACTTGA3514606321.46600730445155No Hit
CAGATC3493207921.335313231916718No Hit
GGGGGG152198579.295765546619206No Hit
ACTAGA1638020.10004463169840028No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)