FastQCFastQC Report
Fri 9 Sep 2022
HVTWYAFX3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTWYAFX3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163728925
Sequences flagged as poor quality0
Sequence length74
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGAT12490090.7628517685558615Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGAT9650610.5894260894951824Clontech Universal Primer Mix Long (100% over 23bp)
GCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCG6180620.3774910267077122Clontech Universal Primer Mix Long (100% over 21bp)
GCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGATCG4869560.2974159880424305Clontech Universal Primer Mix Long (100% over 21bp)
GTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGAG3961820.24197434875969534No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGC3533560.2158176998963378Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAG3474260.2121958597114102Clontech Universal Primer Mix Long (100% over 23bp)
GTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGATCGGAG3146960.19220550064687714No Hit
AGCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATC2932400.17910091329311542Clontech Universal Primer Mix Long (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGCGTAAAGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGTCAAATCC2813420.17183402382932644Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGG2571690.15706998625930024Clontech Universal Primer Mix Long (100% over 23bp)
AGCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGATC2291470.13995511178003522Clontech Universal Primer Mix Long (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGAAGAGCCCAGCTTGAAAATCGGACGTCTTCGGCGTTCGAATTGT2228840.1361298866403722Clontech Universal Primer Mix Long (100% over 23bp)
AGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGGA2003430.12236261857823838No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGTCAAAAGAGGAAAGGCTTGCGGTGGATACCTAGGCACCCAGAGA1983220.12112826124033979Clontech Universal Primer Mix Long (100% over 23bp)
GCAGTGGTATCAACGCAGAGTGAATGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGCTC1952240.1192361093191078Clontech Universal Primer Mix Long (100% over 21bp)
CAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGATCGG1915430.11698788103568139No Hit
GCAGTGGTATCAACGCAGAGTGAATGGGGGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCA1854580.11327137217812919Clontech Universal Primer Mix Long (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATACTAGG1832220.11190570022981583Clontech Universal Primer Mix Long (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)