FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l04_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l04_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71565531
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7340831.0257493932379262No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGACACTCTTTCCCTACACG2752920.3846712183271581Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2118720.29605313764806696No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360730.19013762365572331No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070620.1495999519657026No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030050.14393102176521264No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA972320.13586428919251645No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA927800.12964341730378554No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA867830.12126368488763117No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA839230.11726734760062074No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA715870.10002999907874645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)