FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l04_n02_HTO-CPAcore_K562-L2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l04_n02_HTO-CPAcore_K562-L2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51416001
Sequences flagged as poor quality0
Sequence length101
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3742250.7278376239334522No Hit
CGCCTATATACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3664480.712711982404077No Hit
TCCAATGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3039950.5912459041690155No Hit
GACGTACCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2569770.499799663532759No Hit
TAACGACGTGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2507490.48768670282233734No Hit
GTGTGACGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2226370.4330111165199332No Hit
TAACGACGTGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2042460.39724209589929016No Hit
TATCACATCGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2027770.3943850086668545No Hit
CGCCTATATACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1983650.3858040223703901No Hit
CACATAATGACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1692760.32922824939263556No Hit
ACTGTCTAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1643320.31961256574582686No Hit
CGCCTATATACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1603790.31192429765200913No Hit
TCCAATGCAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510990.29387544161592805No Hit
TAACGACGTGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1475010.28687762006228373No Hit
GTGTGACGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330930.2588552151304027No Hit
TATCACATCGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1310220.2548272861594195No Hit
TCCAATGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1277300.2484246100742063No Hit
ACTGTCTAACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130670.2199062505852993No Hit
CACATAATGACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1123570.2185253575049526No Hit
GACGTACCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1107340.21536875261846988No Hit
GTGTGACGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1084940.21101213219596754No Hit
TATCACATCGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1075540.20918390755438176No Hit
ACTGTCTAACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1023960.19915201106363756No Hit
GACGTACCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA984240.19142678949302183No Hit
CACATAATGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA965630.18780729368664825No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)