FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l04_n02_HTO-CPAcore_K562-L1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l04_n02_HTO-CPAcore_K562-L1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52190950
Sequences flagged as poor quality0
Sequence length101
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCTATATACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3674830.7041124945991595No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3543590.6789663725224392No Hit
TCCAATGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3448440.6607352424127171No Hit
GACGTACCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2680310.5135583851223248No Hit
TAACGACGTGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2612920.5006461848270629No Hit
TAACGACGTGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2173110.41637678563045893No Hit
TATCACATCGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2155860.41307161490641575No Hit
GTGTGACGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2128830.4078925560849151No Hit
CGCCTATATACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2029240.3888107037714393No Hit
TCCAATGCAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1719230.32941151674763536No Hit
ACTGTCTAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1704890.3266639139544308No Hit
CACATAATGACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1687450.3233223384513982No Hit
CGCCTATATACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1627270.31179160371673637No Hit
TAACGACGTGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1577390.30223439121150314No Hit
TCCAATGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1445300.2769254056498301No Hit
TATCACATCGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1382810.26495206544429634No Hit
GTGTGACGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1288650.24691062339351938No Hit
ACTGTCTAACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1172750.22470370820994828No Hit
GACGTACCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1132500.21699164318718092No Hit
TATCACATCGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120100.21461575234786875No Hit
CACATAATGACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1082810.2074708354609372No Hit
GTGTGACGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1066110.20427104699186355No Hit
ACTGTCTAACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1063220.20371731114302385No Hit
GACGTACCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1017550.19496675189855714No Hit
CACATAATGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA928790.1779599719874806No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)