FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l04_n02_ADT-CaRPool_PDL1-L2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l04_n02_ADT-CaRPool_PDL1-L2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214301469
Sequences flagged as poor quality0
Sequence length101
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84778833.9560545429578924No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58681242.738256544568997No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43253652.018355273150274No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42812231.9977571875627227No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39219281.8300985141637083No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38990961.8194443641447928No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36191481.688811568529192No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35893131.674889592100743No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30478331.4222175023914558No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26843601.2526092389968637No Hit
CTCCGAATCATGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26241241.2245011722248158No Hit
ACAGTACGACCTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24624851.149075184360962No Hit
CGCGCACCCATTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24586381.1472800496761877No Hit
ATTCTGTCTTTCCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24465601.141644064045123No Hit
CGCGCACCCATTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23032541.0747728472173934No Hit
CTCCGAATCATGTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22910131.0690608005118247No Hit
CGTCACCTCTGTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22659901.0573842589945102No Hit
CGTCACCTCTGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22471591.048597105043643No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21542611.0052478921644723No Hit
ACAGTACGACCTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21351630.9963361473737729No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20483320.9558179930161842No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16588750.7740847544073531No Hit
ATTCTGTCTTTCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16189770.7554670565510683No Hit
TAGCGAGCCCGTATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14772690.6893415182328965No Hit
ATTCTGTCTTTCCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14061620.6561606910870033No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13283870.6198683593718156No Hit
ACAGTACGACCTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13259670.6187391090632235No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10711990.49985611624528803No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9978480.46562816608597307No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9714440.45330720528098667No Hit
TAGCGAGCCCGTATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9485130.44260685865853766No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT9174290.4281020584137946No Hit
GCTCATTACCCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9031880.4214567469903811No Hit
TAGCGAGCCCGTATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9031060.4214184831369495No Hit
GTTGTCCGACAATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8509740.39709200500160824No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8342570.389291311857503No Hit
GTTGTCCGACAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7932190.37014165311204655No Hit
ACAGCGCCGTATTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7700990.35935311297376127No Hit
GTTGTCCGACAATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7494430.34971435496786074No Hit
AAGTTCACTCTTTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7457980.34801348001958865No Hit
AAGTTCACTCTTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6543350.3053338845754716No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT6070620.2832747730721342No Hit
GCTCATTACCCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5691870.2656010724779493No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5414500.2526580907385194No Hit
TCTCAGACCTCCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5173260.24140105171187604No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5067050.2364449494277615No Hit
GCTCATTACCCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4886630.22802596840808403No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4818890.2248650008087439No Hit
GACAAGTGATCTGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4746980.2215094475157331No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4698590.21925141353090774No Hit
ACAGCGCCGTATTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4574090.21344184066232416No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4498670.20992249941133162No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG4450130.20765746594112242No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4334680.2022701953573636No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4298810.20059638508590905No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4065070.18968932032845748No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3902420.18209954501058506No Hit
TCTCAGACCTCCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3764490.17566328488396876No Hit
ACAGCGCCGTATTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600710.1680207801095381No Hit
GACAAGTGATCTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3546890.1655093647538179No Hit
GACAAGTGATCTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3490350.1628710253964708No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG3387130.15805444618767406No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3334520.15559949334738343No Hit
CGCGCACCCATTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3301110.15404047463622378No Hit
TCTCAGACCTCCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3183150.14853607933037546No Hit
ATTCTGTCTTTCCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3179010.1483428935337816No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT3135740.1463237753167245No Hit
CGCGCACCCATTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3047390.14220107842564533No Hit
CTCCGAATCATGTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2984810.1392808931235091No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA2970420.13860940915901981No Hit
AAGTTCACTCTTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2964890.13835136146453575No Hit
CGTCACCTCTGTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2909050.1357456863723132No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2845230.1327676386576706No Hit
CTCCGAATCATGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2746090.1281414454513142No Hit
ACAGTACGACCTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2744680.12807565028870616No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2739390.1278288017708362No Hit
CGTCACCTCTGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2700470.12601266862991034No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2609500.1217677140607935No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2599600.12130574802546035No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2588550.12079011926885111No Hit
ACAGTACGACCTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2549230.11895532083356834No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2528720.11799825786541855No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2490730.11622552153387246No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2483920.11590774489744632No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2432600.11351298763145669No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2415240.11270291385636745No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2381990.11115136126295057No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2324860.10848549059642704No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2296200.10714812225575551No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2268770.10586814969523145No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2262850.10559190333874939No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2223800.10376970397715753No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2217700.10348505823821487No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG2197730.1025531934174469No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2197590.10254666056442197No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2157630.10068199765816818No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2156720.10063953411350625No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2154980.10055834008305374No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2148770.10026856138816295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)