FastQCFastQC Report
Thu 25 Nov 2021
HVTN5DSX2_l04_n02_ADT-CaRPool_PDL1-L1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVTN5DSX2_l04_n02_ADT-CaRPool_PDL1-L1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202380760
Sequences flagged as poor quality0
Sequence length101
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85608914.2300913387221195No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52848812.6113554470296485No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41617882.0564148489214094No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40331161.9928356826014486No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38432381.8990135228269724No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34701881.7146827593690228No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32033791.5828475987539528No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31997451.5810519735176407No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27191091.343561018349768No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26902011.329277051830421No Hit
ACAGTACGACCTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25521761.2610763987643885No Hit
CTCCGAATCATGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22713521.1223161727429032No Hit
ATTCTGTCTTTCCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22577381.1155892487013093No Hit
ACAGTACGACCTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22087771.0913967315865403No Hit
CGCGCACCCATTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22038221.088948376317986No Hit
CGTCACCTCTGTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21826221.078473072242638No Hit
CGTCACCTCTGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21580691.0663409901217882No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21556311.0651363301531234No Hit
CGCGCACCCATTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20512531.0135612693617715No Hit
CTCCGAATCATGTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20385001.0072597810187096No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18293720.9039258474965703No Hit
ATTCTGTCTTTCCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14965080.7394517146788064No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14789010.7307517769969833No Hit
ACAGTACGACCTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13743540.6790932102438987No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13021670.6434243057492224No Hit
TAGCGAGCCCGTATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13001590.6424321165707649No Hit
ATTCTGTCTTTCCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12948900.6398286082135476No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11042590.5456343774971495No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10790040.5331554244583329No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10612970.5244060749648336No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9249210.457020222673341No Hit
ACAGCGCCGTATTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9244840.45680429305631626No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT8965480.44300060934646157No Hit
TAGCGAGCCCGTATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8348170.41249820388064556No Hit
GTTGTCCGACAATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8322850.411247096809005No Hit
GCTCATTACCCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8199040.40512942040537847No Hit
TAGCGAGCCCGTATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7875430.3891392640288533No Hit
GTTGTCCGACAATACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7749420.38291288163953924No Hit
GTTGTCCGACAATACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7282160.3598247185157324No Hit
TCTCAGACCTCCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7168860.35422636025282245No Hit
AAGTTCACTCTTTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6958270.343820726831938No Hit
AAGTTCACTCTTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6150150.3038900535801921No Hit
ACAGCGCCGTATTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5492790.2714087050567455No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5448390.2692148206183236No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5371360.2654086287649083No Hit
GCTCATTACCCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5191250.25650906736391343No Hit
TCTCAGACCTCCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4842690.2392860862860679No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT4466010.22067364506388848No Hit
GCTCATTACCCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4456550.22020620932543192No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG4440760.21942599681906522No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4422560.21852670184655892No Hit
GACAAGTGATCTGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4326190.21376488555532652No Hit
ACAGCGCCGTATTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4285710.21176469541867518No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4214630.2082525038447331No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT4213790.2082109979229251No Hit
TCTCAGACCTCCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4083790.2017874624050231No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3845580.19001707474564283No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3782010.18687596587738875No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3753930.18548848220552192No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3576170.17670503856196607No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3256320.16090067059734334No Hit
GACAAGTGATCTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3227730.15948798690152166No Hit
GACAAGTGATCTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3192240.1577343617051344No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT3098700.15311238084094556No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG3061560.15127722615529263No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA2959360.14622733900198812No Hit
CGCGCACCCATTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2892230.1429103240841669No Hit
ATTCTGTCTTTCCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2882450.14242707656597398No Hit
ACAGTACGACCTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2784980.13761090728189776No Hit
CGTCACCTCTGTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2737600.13526977564468085No Hit
AAGTTCACTCTTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2703670.13359323287450842No Hit
CGCGCACCCATTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2681510.13249826712776452No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2630980.13000148828376767No Hit
CTCCGAATCATGTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2618440.12938186416534853No Hit
ACAGTACGACCTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2578850.12742565054108898No Hit
CGTCACCTCTGTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2528740.12494962465799614No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2503990.12372668231901095No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2470770.12208522193512862No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2455860.12134849182303692No Hit
CTCCGAATCATGTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2426500.1198977610322246No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2340310.11563895698385558No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2310900.11418575560245944No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2277530.11253688344682568No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2235020.11043638733247171No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2220950.10974116314218803No Hit
CGCGCACCCATTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2212240.10931078626248858No Hit
CTCCGAATCATGTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2164980.10697558404267284No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG2161640.10681054859167444No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2157130.10658770132101492No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA2123280.10491511149577656No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2041560.10087717824560002No Hit
AATAGCGAGCAAGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2026860.10015082461396034No Hit
CGTCACCTCTGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2026390.10012760106247254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)